Biophysical Miscellaneous

Biophysical Miscellaneous

Easy Questions

Biophysical

Biophysical Miscellaneous
  1. When electroporation is used for introducing DNA into mammalian cells ​​
    (P)​ a carrier for DNA is not required ​​
    (Q)​ the lipid bilayer (membrane) interacts with an electric pulse to generate permeation sites
    ​​(R)​ the viability of the cells becomes approximately zero percent
    ​​(S)​ the first step involves absorption of DNA on the cell membrane ​​








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    Electroporation, or electropermeabilization, is a significant increase in the electrical conductivity and permeability of the cell plasma membrane caused by an externally applied electrical field. It is usually used in molecular biology as a way of introducing some substance substance into a cell, such as loading it with a molecular probe, a drug that can change the cell’s function, or a piece of coding DNA. ​

    Correct Option: A

    Electroporation, or electropermeabilization, is a significant increase in the electrical conductivity and permeability of the cell plasma membrane caused by an externally applied electrical field. It is usually used in molecular biology as a way of introducing some substance substance into a cell, such as loading it with a molecular probe, a drug that can change the cell’s function, or a piece of coding DNA. ​

  1. Ultrafiltration process can not be used for








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    Ultrafiltration (UF) is a variety of membrane filtration in which hydrostatic pressure forces a liquid against a semipermeable membrane. Suspended solids and solutes of high molecular weight are retained, while water and low molecular weight solutes pass through the membrane. This separation process is used in industry and research for purifying and concentrating macromolecular (103 - 106 Da) solutions, especially protein solutions. Ultrafiltration is not fundamentally different from microfiltration, nanofiltration or gas separation, except in terms of the size of the molecules it retains. Ultrafiltration is applied in cross-flow or dead-end mode and separation in ultrafiltration undergoes concentration polarization.

    Correct Option: C

    Ultrafiltration (UF) is a variety of membrane filtration in which hydrostatic pressure forces a liquid against a semipermeable membrane. Suspended solids and solutes of high molecular weight are retained, while water and low molecular weight solutes pass through the membrane. This separation process is used in industry and research for purifying and concentrating macromolecular (103 - 106 Da) solutions, especially protein solutions. Ultrafiltration is not fundamentally different from microfiltration, nanofiltration or gas separation, except in terms of the size of the molecules it retains. Ultrafiltration is applied in cross-flow or dead-end mode and separation in ultrafiltration undergoes concentration polarization.

  1. Cibacren Blue dye affinity chromatography can be used for affinity purification of ​​








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    Cibacren blue dye (ligand) used in affinity chromatography, due to presence of sulfonated polyaromatic compounds which bind with considerable specificity and significant affinity to nucleotide-dependent enzymes and to a series of other proteins is used for glucoamylse purification.

    Correct Option: A

    Cibacren blue dye (ligand) used in affinity chromatography, due to presence of sulfonated polyaromatic compounds which bind with considerable specificity and significant affinity to nucleotide-dependent enzymes and to a series of other proteins is used for glucoamylse purification.

  1. Mechanism of separation of contaminants present in air by fibrous media are ​​








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    The fibrous filter media are known to capture the airborne particles of different sizes due to Brownian diffusion, direct interception, inertial impaction, gravity settling, and particle sieving mechanisms.

    Correct Option: D

    The fibrous filter media are known to capture the airborne particles of different sizes due to Brownian diffusion, direct interception, inertial impaction, gravity settling, and particle sieving mechanisms.