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When electroporation is used for introducing DNA into mammalian cells
(P) a carrier for DNA is not required
(Q) the lipid bilayer (membrane) interacts with an electric pulse to generate permeation sites
(R) the viability of the cells becomes approximately zero percent
(S) the first step involves absorption of DNA on the cell membrane
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- P, Q
- Q, R
- P, S
- Q, S
Correct Option: A
Electroporation, or electropermeabilization, is a significant increase in the electrical conductivity and permeability of the cell plasma membrane caused by an externally applied electrical field. It is usually used in molecular biology as a way of introducing some substance substance into a cell, such as loading it with a molecular probe, a drug that can change the cell’s function, or a piece of coding DNA.
