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Biophysical Miscellaneous

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Biophysical

Biophysical Miscellaneous
  1. The helix content of a protein can be determined using ​​
    1. an infrared spectrometer​ ​​
    2. a fluorescence spectrometer ​​
    3. a circular dichroism spectrometer
    4. a UV-Visible spectrophotometer
Correct Option: C

The helix content of a protein can be determined using – circular dichromism spectroscopy. CD spectrum of unknown protein = A *  [% alpha-helical] + B * [% beta-sheet] + C * [% random coil] ​


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