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Match the entries in Group I with the methods of sterilization in Group II.
Group I
P.Serum
Q.Luria broth fïltration
R.Polypropylene tubes
S.Biological safety cabinets
Group II
1.Autoclave
2.Membrane
3.UV irradiation
4.Gamma irradiation
5.Dry heat
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- P - 5 , Q - 3 , R - 1 , S - 4
- P - 1 , Q - 4 , R - 5 , S - 3
- P - 2 , Q - 1 , R - 4 , S - 3
- P - 4 , Q - 1 , R - 3 , S - 5
Correct Option: C
Membrane filtration traps contaminants larger than the pore size on the surface of the membrane. If contaminants are smaller than the desired particle, decrease the membrane pore size and trap the product while passing the contaminants through the membrane. Heat labile fluids such as serum are sterilized by membrane filtration.
A widely used method for heat sterilization is the autoclave. It can be used to sterilize bacterial cultures like Luria Broth.
Gamma irradiation is a physical means of sterilisation or decontamination where products are exposed to gamma rays. It can be used to sterilize polypropylene tubes.
In UV irradiation, Ultraviolet lamps are used to sterilize workspaces and tools used in biology laboratories and medical facilities. It can be used to sterile Biological safety cabinets.
