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If the dissociation constant for solute-adsorbent binding is KD, the retention time of the solute in a chromatography column
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- increases with increasing KD
- decreases with increasing KD
- passes through minimum with increasing KD
- is independent of KD
Correct Option: B
Chromatography involves a sample (or sample extract) being dissolved in a mobile phase (which may be a gas, a liquid or a supercritical fluid). The mobile phase is then forced through an immobile, immiscible stationary phase. The distribution of analytes between phases can often be described quite simply. An analyte is in equilibrium between the two phases ;
Amobile ⇔ Astationary
The time between sample injection and an analyte peak reaching a detector at the end of the column is termed the retention time (tR).
The dissociation constant for solute-adsorbent binding is KD. So, KD is indirectly proportional to the retention time of the column. So, retention time (tR) of a column decreases with increasing KD value.
