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How many λ-phage vectors/yeast clones should be prepared in order to ensure that every sequence is included in the library?
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- 25 × 103 λ-phage vectors/2000 yeast clones
- 20 × 103 λ-phage vectors/1600 yeast clones
- 5 × 104 λ-phage vectors/4000 yeast clones
- 10 × 104 λ-phage/10000 yeast clones
Correct Option: C
λ-phage vectors
| = | = | |||||
| in(1 - a / b) | in |  | 1 - |  | ||
| 108 bp | ||||||
| = | = | ||
| in(1 - 0.0002) | in(0.9998) |
| = | = 5 × 104 | |
| -2.0 × 104 |
| Yeast clones = | ||||
| in | | 1 - | | |
| 108 | ||||
| = | ||
| in(1 - 0.0025) |
| = | ||
| in(0.9975) |
| = | = 4000 | |
| -2.5 × 10-3 |
