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Advanced Biotech Miscellaneous

  1. Mung bean nuclease could be used for ​​
    1. DNA synthesis​ ​​
    2. nucleotide hydrolysis
    3. ​trimming single stranded regions in DNA ​​
    4. removal of phosphate groups from the ends of the DNA
Correct Option: C

Mung Bean Nuclease acts as a preferential DNA and RNA endonuclease, hydrolyzing single-stranded DNA and RNA into 5’-phosphate and 3’-hydroxyl-containing products. If used at very high concentrations, Mung Bean Nuclease is also capable of degrading double-stranded DNA, RNA or DNA/RNA fragments from both ends. Due to its single-stranded DNA and RNA endonuclease activity, Mung Bean Nuclease is often used during transcript mapping, duplex or hybrid DNA and RNA blunt ending, and cDNA preparation.



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